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KMID : 0355820130340010017
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Korean Journal of Oral Anatomy
2013 Volume.34 No. 1 p.17 ~ p.27
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Effect of Bone Morphogenetic Protein-4 on the Proliferation and Differentiation of Cultured Human Osteoblasts and Periodontal Ugament Fibroblasts
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Bang Hee-Joon
Kim Ji-Youn
Jue Seong-Suk
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Abstract
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This experiment was designed to elucidate the effect of bone morphogenetic protein CBMP)-4 on the proliferation, a lka line phosphatase activity , and protein synthesis of human periodontal ligament fibroblasts and osteoblasts. The cells were incubated 24 and 48 hours after application of BMP-4 at concentrations of 50 and 100 ng/ml. After incubation , CCK-8 , alka line phosphatase , and total protein assays were performed. Cell proliferation activity was increased by BMP- 4 application both in periodontal ligament cells and osteoblasts. The cell proliferation activity of the periodontal ligament cells was greater than that of the osteoblasts. Alkaline phosphatase activity was increased by BMP-4 application both in periodontal ligament cells and osteoblasts. The Alkaline phosphatase activity of the osteoblasts was greater than that of the periodontal ligament cells. Protein synthesis was increased by BMP-4 application both in periodontal ligament cells and osteoblasts. The protein synthesis of the periodontal ligament cells was as much as that of the osteoblasts. These results suggest that BMP-4 may promote the periodontal tissue regeneration by stimulating the cell proliferation, alkaline phosphatase activity, and protein synthesis of the periodontal ligament cells and osteoblasts.
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KEYWORD
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BMP-4, osteoblast, PDL cell, differentiation
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